Application

Suitable for immunocytochemistry, immunoprecipitation and western blotting at a working concentration of 30-40 µg/mL using total cell extract of mouse heart.

Biochem/physiol Actions

CUGBP2 is an RNA-binding protein that specifically binds to CUG repeat sequences and is responsible for the regulation of both nuclear and cytoplasmic RNA processing events such as alternative splicing, RNA editing, mRNA stability, and translation. It is mainly expressed in muscle and brain. It regulates a specific alternative slicing of exons 5 and 21 of the N-methyl-D-aspartate receptor 1 (NMDA R1) RNA in rat. It binds to U/G-rich motifs within muscle specific enhancer elements (MSEs) and activates inclusion of an alternative exon in cardiac troponin T (cTNT) pre-mRNAs. It interacts with A/U rich sequences in the 3′ UTR and inhibits translation of cycloxygenase-2 (COX-2) mRNAs.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

divergent domain of human CUGBP2 corresponding to amino acids 209-415 (accession no. NP006552).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Target description

CUGBP2 is an RNA-binding protein that specificallybinds to CUG repeat sequences.