Anti-NRL (Neural Retina Leucine Zipp

Application

Research CategoryNeuroscience

Immunofluorescence Analysis: A 1:100-1:1,000 dilution from a representative lot detected NRL (Neural Retina Leucine Zipper) in frozen mouse retina tissue (Courtesy of Dr. Tiansen Li at NIH).

Western Blotting Analysis: A 1:1,000-1:2,000 dilution from a representative lot detected NRL (Neural Retina Leucine Zipper) in mouse retinal tissue lysate (Courtesy of Dr. Tiansen Li at NIH).

Anti-NRL (Neural Retina Leucine Zipper), Cat. No. ABN1712, is a highly specific rabbit polyclonal antibody that targets Neural retina-specific leucine zipper protein and has been tested for use in Immunofluorescence and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neural retina-specific leucine zipper protein (UniProt: P54846; also known as NRL) is encoded by the Nrl gene (Gene ID: 18185) in murine species. NRL is a bZIP transcription factor of the MAF family that regulates the expression of several rod-specific genes, including RHO and phosphodiesterase 6B. NRL is reported to be present in the nuclei of developing and mature rods but not cones. It interacts with the homeodomain protein CRX and synergistically regulates rhodopsin expression. It is also reported to interact with FIZ1 and this interaction can repress transactivation. It consists of a minimal transactivation domain (aa 30-93), a basic motif (aa 159-185), and a leucine-zipper (aa 187-208). The minimal transactivation domain (MTD) is conserved across the MAF family and it may activate transcription by recruiting TBP and associated factors at the promoters of target genes. In developing mouse retina, Nrl expression is reported to precede the appearance of known markers for differentiated rods. The transcriptional activity of NRL is modulated by sumoylation on RHO and NR2E3 promoters. (Ref.: Prabodha, K et al. (2001). J. Biol. Chem 276 (39): 36824-30).

Immunogen

His-tagged recombinant protein fragment corresponding to 108 amino acids from the N-terminal half of murine neural retina-specific leucine zipper protein.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse retinal tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NRL (Neural Retina Leucine Zipper) in mouse retinal tissue lysate.

Specificity

This rabbit polyclonal antibody recognizes murine neural retina-specific leucine zipper protein. It iargets an epitope within 108 amino acids from the N-terminal half.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~31 kDa observed; 26.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).