Analysis Note

ControlRaji cell nuclear extract

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated ARID1B from Raji cell nuclear extract.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

This Anti-ARID1B Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of ARID1B.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ARID1B (AT rich interactive domain 1B), also known as BAF250B, p250R and hOsa2, is a nuclear protein and a member of the SWI/SNF-A chromatin-remodeling complex. It is involved in repression of select genes by chromatin remodeling and transcriptional activation. It non-specifically binds DNA and alters DNA nucleosome topology. The cause of mental retardation autosomal dominant type 12 (MRD12) are defects in ARID1B. High levels of ARID1B can be found in heart, skeletal muscle and kidney.

Immunogen

KLH-conjugated linear peptide corresponding to human ARID1B.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in Raji cell nuclear extract.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ARID1B in 10 µg of Raji cell nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~250 kDa observed. Uncharacterized bands may be observed at ~16 kDa and ~32 kDa in some lysates.. Uniprot describes 4 isoforms produced by alternative splicing at ~236 kDa, ~237 kDa, ~241 kDa and ~162 kDa This antibody will recognize all isoforms.