Analysis Note

ControlA431 cell lysate

Application

Anti-JARID1B Antibody is a Rabbit Polyclonal Antibody for detection of JARID1B also known as lysine (K)-specific demethylase 5B & has been validated in WB & IP.

Immunoprecipitation:
A representative lot of this antibody was reported to work for immunoprecipitation analysis in Saos-2, MCF-7, and T47D lysates (Laboratory of E. Benevolenskaya , University of Illinois at Chicago).

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

Research Sub CategoryChromatin BiologyHistone Modifying Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

JARID1B (jumonji AT rich interactive domain 1B), also known as KDM5B, is histone demethylase with a preference for mono, di, and trimethylated lysine 4 of histone H3. Demethylation of lysine 4 in histone 3 is commonly associated with transcriptional repression. This nuclear protein belongs to the ARID family of DNA binding proteins and is highly expressed in a large number of breast cancers. This protein has been linked to the repressinion of multiple tumor suppressor genes including BRCA1 and HOXA5.

Immunogen

Epitope: C-terminal domain

GST-tagged recombinant protein corresponding to human JARID1B at the C-terminal domain.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected JARID1B on 10 µg of A431 cell lysate.

Specificity

This antibody recognizes JARID1B at the C-terminal domain.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~180 kDa observed. Uncharacterized bands may be observed at ~ 48 and 53 kDa in some lysates.