Analysis Note

ControlA431 cell lysate

Application

Research Sub CategoryTranscription Factors

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated β-Catenin from A431 lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected β-Catenin in NIH/3T3 and A431 cells.

Research CategoryEpigenetics & Nuclear Function

Anti-β-Catenin Antibody is a Rabbit Polyclonal Antibody for detection of β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD) & has been validated in WB, ICC & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

β-catenin is the founding member of the β -catenin family. This protein is comprised of 12 ARM repeats and is considered to have dual function depending on its localization. Cytoplasmic β -catenin is a critical component of E-cadherin/catening adhesion complexes and is involved in regulating cadherin-dependent cell-cell interactions.When localized to the nucleus, β -catenin serves a regulatory role by binding with transcription factors from the TCF/LEF family, activating transcription. Expression of β -catenin has been reported in a variety of hair follicle cells such as cells found in the inner and outer portions of the root sheaths, and the basal and peripheral matrix cells. It has also been observed in colon tissue. Defects in expression have been linked to the development of several tumors and cancers such as; colorectal cancer (CRC), medulloblastoma (MDB), ovarian cancer (OC), and pilomatrixoma (PTR).

Immunogen

KLH-conjugated linear peptide corresponding to the consensus GSK3 phosphorylation site of human β-Catenin.

Epitope: Consensus GSK3 phosphorylation site of human β-Catenin

Linkage

Replaces: 04-1002

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected β-Catenin on 10 µg of A431 cell lysate.

Specificity

Antibody recognizes β-Catenin at the N-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~86kDa observed.