Application

Research CategoryEpigenetics & Nuclear Function

Anti-CDK13, Cat. No. ABE1860, is a highly specific rabbit polyclonal antibody that targets Cyclin-dependent kinase 13 and has been tested in Western Blotting.

Western Blotting Analysis: A representative lot detected CDK13 in HeLa cell lysate (Bartkowiak, B., et. al. (2010). Genes Dev. 24(20):2303-16).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HEK293 whole cell extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HeLa nuclear extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in OVCA5 and HEK293 (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cyclin-dependent kinase 13 (UniProt: Q14004: also known as Cdk13, EC:2.7.11.22, EC:2.7.11.23, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, Cell division protein kinase 13, hCDK13, Cholinesterase-related cell division controller) is encoded by the CDK13 (also known as CDC2L, CDC2L5, CHED, KIAA1791) gene (Gene ID:8621) in human. CDK13 is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are known to play a vital role as master switches in cell cycle control. Human CDK 13 shares 43% sequence identity with CDK12. CDK13 is expressed in fetal brain, liver, muscle, in adult brain and in neuroblastoma and glioblastoma tumors. It contains expanded regions of serine-arginine (SR) motifs in its N-terminal regions, which links it to the SR protein family involved in RNA processing and pre-mRNA splicing. CDK13 contains a C-terminal extension helix that is composed of a polybasic cluster and a DCHEL motif that interacts with the bound ATP. CDK13 displays C-terminal repeat domain (CTD) kinase activity and is required for RNA splicing. It hyperphosphorylates the C-terminal heptapeptide repeat domain of the largest RNA polymerase II subunit RPB1 and acts a key regulator of transcription elongation. Phosphorylation of the three serine residues (Ser 2, 5, and 7) within the CTD is linked to the phases of RNAPII-mediated transcription. Phosphorylation of Ser7 (pSer7) starts the transcription cycle that primes the CTD for further modifications. (Ref.:Greifenberg, AK et al. (2016). Cell Reports 14(2): 320-331).

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Cyclin-dependent kinase 13 (CDk13).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 whole cell extract.

Western Blotting Analysis: 1:500 dilution of this antibody detected CDK13 in 20 µL of HEK293 whole cell extract.

Specificity

This polyclonal antibody specifically detects CDK13 in HEK293 cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~170 kDa observed; 164.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).