Application

Research Sub CategoryNuclear Receptors

This Anti-phospho Histone H3 (Tyr41) Antibody is validated for use in Western Blotting and Dot Blot and Immunohistochemistry for the detection of phospho Histone H3 (Tyr41).

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Tyr41) in human testis and human prostate tissue.
Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected phospho Histone H3 (Tyr41) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

Immunogen

KLH-conjugated linear peptide corresponding to human phospho Histone H3 (Tyr41) near the N-terminus.

Epitope: Near N-terminus

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in colcemid treated HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho Histone H3 (Tyr41) in 10 µg of colcemid treated HeLa acid extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed. Uncharacterized band(s) may appear in some lysates.