Application

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

Immunofluorescence Analysis: A representative lot detected Lhx2 immunoreactivity by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed backskin cryosections from wild-type, but not Lhx2 knockout, mice at varies developmental stages, including E16.5, P0, P30, and the second telogen (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions using A6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions by a genome-wide ChIP-seq analysis using A6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Immunofluorescence Analysis: A representative lot (1:1,000 dilution) detected Lhx2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University ).

This Anti-LHX2 Antibody is validated for use in Western Blotting, Immunofluorescence, ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of LHX2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

LIM/homeobox protein Lhx2 (UniProt Q9Z0S2; also known as Homeobox protein LH-2, LIM homeo box protein 2, Lim2) is encoded by the Lhx2 gene (Gene ID 16870) in murine species. Lhx2 is a transcription factor that plays an important role in controlling the behavior of progenitor cells involved in the development of the skin hair cycling, eye, forebrain, limb, hematopoietic, olfactory, and pituitary systems. Lhx2 is shown to regulate a significant number of hair follicle stem cell (HF-SC) signature genes, as well as cytoskeletal and adhesion molecules within the niche where HF-SCs reside as a single layer of polarized, quiescent epithelial cells. Lhx2 deficiency leads to baldness, because the niche can no longer anchor its hair and maintain the proper behavior of its SCs as a result of greatly perturbed HF-SCs polarization and niche architecture.

Immunogen

Recombinant protein corresponding to mouse LHX2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected doxycycline-induced LHX2 expression in 10 µg of Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~48 kDa detected. Target band size appears larger than the calculated molecular weight of 44.4 kDa due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.