General description

Note: 1 mU = 1 milliunit.

Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore, is more suitable for deglycosylation of native proteins.

Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Reddy, A., et al. 1998. Glycobiology 8, 633.Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Packaging

200 miu in Plastic ampoule

Physical form

In 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.

Unit Definition

One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.

Warning

Toxicity: Standard Handling (A)