04-745 Display Image

A-TRIMETH-HISH3 (K4);CL MC315 100

Code: 04-745 D2-231

Analysis Note

ControlHeLa acid extract

Application

Research Sub CategoryHistones

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution...


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Analysis Note

ControlHeLa acid extract

Application

Research Sub CategoryHistones

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections

Research CategoryEpigenetics & Nuclear Function

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. r> r>The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-791

Physical form

Cultured supernantant in 0.05% sodium azide.

Quality

routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)

Specificity

Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

17 kDa

antibody formculture supernatant
antibody product typeprimary antibodies
biological sourcerabbit
cloneMC315, monoclonal
Gene Informationhuman ... H3C1(8350)
isotypeIgG
manufacturer/tradenameUpstate®
NCBI accession no.NM_003493
Quality Level100
shipped indry ice
species reactivityhuman
technique(s)western blot: suitable, immunohistochemistry: suitable (paraffin), ChIP: suitable (ChIP-seq), multiplexing: suitable, dot blot: suitable
UniProt accession no.Q16695
This product has met the following criteria: