Application

Sephadex^®G-50 has been used to purify the DNA products in cycle sequencing reactions.

Sephadex^® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.

Features and Benefits

Excellent recovery Minimal sample dilution Quick and easy to use

General description

Sephadex^® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Legal Information

Sephadex is a registered trademark of Cytiva

Packaging

25, 100 g in poly bottle