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28401748 Display Image
28401748 Display Image

GSTrap 4B 5 x 5 ml

Code: 28401748 D2-84

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

  • High binding capacity.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
  • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA design.

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.

GSTrap 4B 5 x 1mLGSTrap 4B 1 x 5mLGSTrap 4B 5 x 5mL
ApplicationGSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Bed dimensions, mm7 x 2516 x 2516 x 25
Bed height, mm252525
Bed volume, mL155
Column i.d.71616
Flow rate, mL/min<4<20<20
Storage4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol4 to 30°C, 20% Ethanol
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)
Chromatography techniqueGST-tagged protein purificationGST-tagged protein purificationGST-tagged protein purification
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