MabSelect SuRe LX is a protein A affinity resin with very high dynamic binding capacity at extended residence times. The chromatography resin is developed for processing of monoclonal antibodies at high titres.

• Novel, alkali-tolerant rProtein A ligand withstands rigorous clean-in-place and sanitisation procedures with 0.1 to 0.5M NaOH
• High dynamic binding capacity (DBC) for high titre cultures reduces process time and amount of resin used
• Fulfils industrial demands for security of supply, robust performance, and regulatory support

Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matrix makes MabSelect SuRe LX an excellent choice for purification of monoclonal antibodies for clinical applications.

MabSelect SuRe LX is a member of the MabSelect family of protein A resins for the capture of monoclonal antibodies at process scale. MabSelect SuRe LX shares features and benefits of MabSelect and MabSelect SuRe such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for excellent binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect SuRe. This protein ligand provides greater stability than conventional protein A-based resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitisation protocols.

The enhanced alkali stability of MabSelect SuRe LX improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect SuRe LX.

Just as for MabSelect SuRe, the novel ligand construct of MabSelect SuRe LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect SuRe LX ligand during elution is low.

MabSelect SuRe has been demonstrated to give generic elution conditions for different monoclonal antibodies, which can be applied also to MabSelect SuRe LX as it utilises the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect SuRe protein ligand.