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17040301 Display Image
17040301 Display Image

HiTrap Protein A HP 1 x 5 ml

Code: 17040301 D2-84

HiTrap Protein A HP are prepacked columns for routine preparative purification of monoclonal and polyclonal antibodies such as human IgG.

  • First choice Protein A resin for routine ...


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    £840.00 EACH
    £1,008.00 inc. VAT

HiTrap Protein A HP are prepacked columns for routine preparative purification of monoclonal and polyclonal antibodies such as human IgG.

  • First choice Protein A resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
  • Small bead size (34 µm) ensures narrow elution peaks containing concentrated material

Protein A column for IgG purification

HiTrap Protein A HP is packed with Protein A Sepharose High Performance resin. Use this column for routine immunoaffinity purifications of most IgGs from ascites, serum, and cell culture supernatant.

These columns deliver high-performance, high-resolution purifications with a binding capacity of 20mg IgG/mL resin. Columns are stable over a wide pH range – operational pH 3 to 9 for use; pH 2 to 10 for cleaning.

HiTrap Protein A HP 5 x 1mLHiTrap Protein A HP 2 x 1mLHiTrap Protein A HP 1 x 5mLHiTrap Protein A HP 5 x 5mL
Bed dims, mm7 x 257 x 2516 x 2516 x 25
Bed height, mm25252525
Bed volume, mL1155
Column i.d.771616
Flow rate, mL/min<4<4<20<20
ApplicationCan be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)
Storage4 to 8°C, 20% Ethanol4 to 8°C, 20% Ethanol4 to 8°C, 20% Ethanol4 to 8°C, 20% Ethanol
Chromatography techniqueAntibody affinity chromatographyAntibody affinity chromatographyAntibody affinity chromatographyAntibody affinity chromatography
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17040203
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17040201
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17040303
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