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NHS Mag Sepharose 4x500ul

Code: 28951380 D2-84

NHS Mag Sepharose magnetic beads are designed for pull-down techniques, involving isolation of a protein through adsorption of complex samples onto beads, enabling rapid capture and enrichment of s...


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£152.00 EACH
£182.40 inc. VAT

NHS Mag Sepharose magnetic beads are designed for pull-down techniques, involving isolation of a protein through adsorption of complex samples onto beads, enabling rapid capture and enrichment of selected proteins based on affinity.

  • Visible and dense Sepharose based magnetic beads with N-hydroxysuccinimide ligand for easy coupling of biomolecules containing a free amine group.
  • Non-adherent beads eliminate smearing effects and aggregate formation.
  • Can be used without detergents.
  • Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale).
  • Optimized capacity for enrichment or immunoprecipitation of target proteins, only low amounts of antibody needed.

Flexible protocols with elution conditions optimized for both electrophoresis and mass spectrometry (MS) analysis of antibodies or other proteins with the desired affinity are covalently coupled to the medium via primary amines for purpose of target proteins of interest.

Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.

NHS Mag Sepharose
Binding Capacity8 - 14µmole/ml gel
ApplicationDesigned for covalent coupling of any protein or biomolecule containing a free amine group and is used for various types of pull-down experiments. Used for biomarker research
MatrixParamagneticm, spherical, highly cross-linked agarose particles
LigandN-hydroxysuccinimide
Operating TemperatureRoom temperature
Storage Conditions4-8°C, 2-propanol, 20% medium slurry
StabilityStable
Chromatography TechniqueAffinity coupling
Bead TypeMag Sepharose Magnetic Beads

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