Application

Research CategorySignaling

Detect Src-like-adapter 2 using this mouse monoclonal Anti-Src-Like-Adaptor 2 (SLA2) antibody, clone Z30P1F12*F4, Cat. No. MABS1922, validated for use in Immunohistochemistry (Paraffin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Src-like-adapter 2 (UniProt: Q9H6Q3; also known as Modulator of antigen receptor signaling; MARS; Src-like adapter protein 2; SLAP-2) is encoded by the SLA2 (also known as SLAP2, C20orf156) gene (Gene ID 84174) in human. SLAP-2 is localized to the plasma membrane and intracellular vesicles, including late endosomal vesicles. The SLAP-2 protein contains an NH2-terminal myristoylation consensus sequence and SH3 and SH2 Src homology domains, but lacks a tyrosine kinase domain. It is predominantly expressed in immune system, including T cells and monocytes and at low levels in B cells, placenta, prostate, colon, and skin. SLAP-2 serves as an adapter protein that negatively regulates T-cell receptor (TCR) signaling and is shown to block T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. In antigen receptor-stimulated cells, SLAP-2 associates with several tyrosine phosphorylated proteins, including the ubiquitin ligase Cbl. Deletion of the carboxyl terminus of SLAP-2 is shown to block its function and abolish its association with Cbl. Overexpression of SLAP-2 in T and B cell lines impairs antigen receptor-mediated signaling events, including the up-regulation of CD69, NFAT promoter activation, and calcium influx. Ref.: Holland, SJ et al. (2001). J. Exp. Med. 194(9):1263-1276. Pandey, A., et al. (2002). J. Biol. Chem. 277(21):19131-19138.

Immunogen

A synthetic peptide corresponding to 8 aa from the C-terminal region of human Src-like adapter 2 protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry in human cervical cancer, pancreas, and tonsil tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Src-Like-Adaptor 2 (SLA2) in human cervical cancer, pancreas, and tonsil tissue.

Specificity

Clone Z30P1F12*F4 detects -Src-Like-Adaptor 2 (SLA2) protein in human by targeting a sequence in the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

28.6 kDa calculated.