Application

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Features and Benefits

Fast and easy-to-use Up to 20x more sensitive than conventional buffers Doesn’;t require blocking steps Compatible with neutral and positively-charged membranes Compatible with chemiluminescent, colorimetric , and DIG detection assays Compatible with radioactive and non-radioactive probes Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

General description

Sigma’;s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.