Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Primary TargetUsed as a direct indicator of cytotoxicity and apoptosis.

General description

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Liu, Y., et al. 1997. J. Neurochem.69, 581.Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

Packaging

1 g in Glass bottle

10 g in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)