Biochem/physiol Actions

Product does not compete with ATP.

Primary TargetSerine, cysteine, metalloproteinases, and calpain proteases

General description

A protease cocktail specifically developed for 2D gel sample preparation.

A protease cocktail specifically developed for 2D gel sample preparation. Inhibits serine, cysteine, metalloproteinases, and calpain proteases from animal tissues, plants, yeast, and bacteria. FOCUS-Protease Arrest™ Cocktail at 1X concentration in extraction buffer, pH 7.0 - 8.0, inhibits 95-98% of protease activity.

A protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease™ for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest™ contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest™ contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest™, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 ml in Plastic ampoule

Physical form

Supplied as 100X.

Reconstitution

Following dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.

Warning

Toxicity: Irritant (B)