Biochem/physiol Actions

Reversible: yes

Product does not compete with ATP.

Primary TargetATPase

Cell permeable: yes

Target IC₅₀: ﹤100 µM inhibiting the ATPase and gliding motility of human platelets

General description

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.Straight, A.F., et al. 2003. Science299, 1743.Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Physical form

A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203390) in 90% DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)