Biochem/physiol Actions
Reversible: yes
Product does not compete with ATP.
Primary TargetATPase
Cell permeable: yes
Target IC50: ﹤100 µM inhibiting the ATPase and gliding motility of human platelets
General description
A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.Straight, A.F., et al. 2003. Science299, 1743.Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.
Packaging
Packaged under inert gas
5 mg in Plastic ampoule
Physical form
A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203390) in 90% DMSO.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
This product has met the following criteria: