Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Primary TargetPKC

Target K_i: 10 nM against PKC

Cell permeable: yes

General description

A highly selective cell-permeable protein kinase C (PKC) inhibitor (K_i = 10 nM) that is structurally similar to staurosporine. Acts as a competitive inhibitor for the ATP-binding site of PKC. Shows high selectivity for PKCα, βI, βII, γ, δ, and ε isozymes. May inhibit protein kinase A at a much higher concentration (K_i = 2 µM). Also inhibits telomerase A treated NPC-076 cells. A 1 mg/ml solution of Bisindolylmaleimide I (Cat. No. 203290) in anhydrous DMSO.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ku, W.-C., et al. 1997. Biochem. Biophys. Res. Commun.241, 730.Gekeler, V., et al. 1996. Br. J. Cancer74, 897.Kiss, Z., et al. 1995. Biochim. Biophys. Acta1265, 93.Muid, R.E., et al. 1991. FEBS Lett.293, 160.Toullec, D., et al. 1991. J. Biol. Chem.266, 15771.

Packaging

Packaged under inert gas

1 ml in Glass bottle

Physical form

A 1 mg/ml solution of Bisindolylmaleimide I (catNO=203290">Cat. No. 203290) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)