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Anti-alpha-Tubulin; clone RM113

Code: MABT522 D2-231

Application

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Immunocytoch...


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£472.00 EACH
£566.40 inc. VAT

Application

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.

General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Immunogen

A peptide corresponding to the C-terminus of α-Tubulin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.

Target description

~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).

antibody formpurified antibody
antibody product typeprimary antibodies
biological sourcerabbit
cloneRM113, monoclonal
Gene Informationhuman ... TUBA1A(7846)
isotypeIgG
Quality Level100
shipped inwet ice
species reactivityhuman
technique(s)immunocytochemistry: suitable, western blot: suitable
UniProt accession no.P68363
This product has met the following criteria: