Application

Trehalase has been used:in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leavesin a study to investigate growth arrest by trehalose-6-phosphateto quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Physical form

Solution in 50% glycerol containing 1% Triton^™ X-100 and 25 mM potassium phosphate, pH 6.5

Unit Definition

One unit will convert 1.0 µmole of trehalose to 2.0 µmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).