Analysis Note

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

Application

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.

Biochem/physiol Actions

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.

TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.

Other Notes

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

Physical form

Lyophilized from 0.2 µm filtered solution in phosphate buffered saline.

Reconstitution

Reconstitute to 100 µg/mL in phosphate buffered saline.