Sumo-1/sentrin-1 (gst-tagged)

Application

SUMO-1 human has been used in pre-absorption for negative immunostaining to confirm the specificity of the small ubiquitin like modifier 1 (SUMO-1) antibody.

Biochem/physiol Actions

Small ubiquitin-like modifier 1 (SUMO-1) plays a key role in modulating survival, migration, and signal transduction in some cell lines. It can prevent in vitro migration and invasion of RA fibroblast-like synoviocytes (FLSs). Higher expression of SUMO-1 may result in joint destruction in rheumatoid arthritis (RA).

Conjugated to a variety of proteins by UbcH9, including RanGAP1, IκBα, and PML without the requirement of an equivalent E3 ubiquitin ligase.

General description

Small ubiquitin-like modifier 1 (SUMO-1), a protein, is one of the 5 SUMO paralogs expressed in mammalian cells with a low but significant homology with ubiquitin. This SUMO-1-GST fusion protein is produced from a DNA sequence corresponding to human SUMO-1 fused to a glutathione S-transferase (GST)-tag and is expressed in E. coli cells.

Physical form

Solution in 50 mM HEPES, pH 8.0, 150 mM NaCl and 1 mM DTT.