P9529-1UN Display Image

Phosphodiesterase, 3,5-cyclic-nucleotide-specific from bovine brain, 15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level (10 units per ml) of calmodulin (P2277)), lyophilize

Code: P9529-1UN D2-231

Analysis Note

Protein determined by Warburg-Christian

Application

Phosphodiesterase, 3',5'-cyclic-nucleotide-specific from bovine brain has been ...

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Analysis Note

Protein determined by Warburg-Christian

Application

Phosphodiesterase, 3',5'-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

General description

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.

Packaging

Package size based on activated units

Physical form

Lyophilized powder containing Tris-HCl buffer salts and lactose

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

Unit Definition

One unit will hydrolyze 1.0 µmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Gene Informationcow ... PDE1A(281969)
Quality Level200
compositionProtein, 4.0-6.0% Lowry
activity1-5 units/mg protein (without added calmodulin)
foreign activity5′-ATPase ≤0.2%
foreign activity2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%
foreign activityinorganic pyrophosphatase ≤0.8%
mol wt~60 kDa
formlyophilized powder
storage temp.−20°C

Cas Number9040-59-9