Application

4-nitrophenyl butyrate has been used:as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethyleneas a substrate for mycobacterial phospholipase A and mycobacterial cutinase activityas a substrate to determine the effect of buffers and solvents on human carboxylesterase

General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.