Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)to treat adherent cells in adhesion assayas a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to studyon alkaline phosphatases, acid phosphatases etc.

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Packaging

1, 5 g in poly bottle