Pyrimidine Nucleoside Phosphorylase from Bacillus subtilis, recombinant, expressed in E. coli, >=70 units/mg protein

Biochem/physiol Actions

Pyrimidine nucleoside phosphorylase functions in the nucleotide synthesis salvage pathway by catalyzing the reversible phosphorolysis of pyrimidines. Both uridine and thymidine are substrates.

General description

The roles of the residues in the catalytic active site of pyrimidine nucleoside phosphorylase from Bacillis subtilis have been elucidated using hybrid quantum-mechanical/molecular-mechanical methods.

Packaging

250, 1000, 5000 units in poly bottle

Unit Definition

One unit will convert 1 µmole each of 2′-deoxyuridine and phosphate to uracil and 2-deoxyribose 1-phosphate per minute at pH 7.4 and 37 °C.