Application

Mycoplasma PCR Detection Kit Video Protocol

The LookOut^® Mycoplasma PCR Detection Kit has been used for screening of mycoplasma infection.

Compatibility

The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3’;-5’; exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

General description

The LookOut^® Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals. Kit has been successfully tested for the ability to amplify Mycoplasma fermentans genomic DNA in concentrations of 20,000 to 20 copies.The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. Total assay time is greatly reduced compared to general protocols that require individual loading of reaction tubes.

Legal Information

LookOut is a registered trademark of Sigma-Aldrich Co. LLC

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