Application

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Biochem/physiol Actions

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

General description

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Physical form

Suspension in 2.9 M (NH₄)₂SO₄ solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na₄EDTA, pH 6.0

Unit Definition

One unit will convert 1.0 µmole of L-malate and NADP to pyruvate, CO_2, and NADPH per min at pH 7.4 at 25 °C.