Application

A working concentration of 0.1-1.0 µg/mL is recommended for detection of human Lck protein by immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The signalling in T cells is mediated by receptor and non-receptor protein tyrosine kinases (PTK). Among the family of non-receptors PTK, Lck is a lymphoid-specific cytosolic PTK essential for T-cell development and function. Lck is essential for selection and maturation of developing T cells in the thymus. It plays a significant role in T-cell receptors mediated signalling by constitutive association with CD4 and CD8 receptors. In addition, Lck undergoes phosphorylation and activation by association with CD2 molecule. Lck also plays a role in T-cell proliferation in response to IL-2 receptor pathway. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, tyrosine 505 (equivalent to tyrosine 529 in c-Src) and tyrosine 394 (equivalent to tyrosine 418 in c-Src). Phosphorylation at Tyr505 stabillizes Lck and renders it biologically inactive. Anti-phospho Lck (pTyr⁵⁰⁵) specifically recognizes human Lck (pTyr⁵⁰⁵). Mouse (100% homology) and chicken (91.7% homology) are expected to react.

Immunogen

synthetic phosphopeptide derived from the region of Lck that is phosphorylated on tyrosine 505. Rat and mouse have 100% homology with human.

Physical form

Solution in phosphate buffer, pH 7.4, with 1 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Preparation Note

The antibody is preadsorbed to remove any reactivity towards a non-phosphorylated Lck.