Application

Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.

For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

Reconstitution

Reconstitute to 40 mL with deionized water.