Analysis Note

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Application

HiTrap^® Albumin & IgG Depletion and Albumin & IgG Depletion SpinTrap^™ are prepacked columns for the depletion of albumin and IgG from plasma and serum samples. Albumin and IgG are the most abundant plasma Proteins, and they tend to obscure the signals of less abundant Proteins. Depletion of the two Proteins removes more than 60% of the total Protein content in human plasma, allowing a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins. These columns are members of the Trap platform and address the need for flexible, small-scale preparation of Protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.

Features and Benefits

HiTrap^® Albumin & IgG Depletion is a prepacked column for the depletion of albumin and IgG from human serum or plasma. HiTrap^® Albumin & IgG Depletion column provides rapid and easy processing in a convenient format for handling larger sample volumes (~150 µL) using a liquid chromatography system or manually using a syringe. Columns are prepacked with high performance Sepharose^® based media with an affinity for human serum albumin (HSA) and IgG. High depletion capacity, removes > 95% HSA and > 90% IgG, with high reproducibility. Simple and fast procedure. Removal of albumin and IgG allows a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins.

General description

HiTrap^® Albumin & IgG Depletion is prepacked columns for the depletion of albumin and IgG from human serum and plasma. The columns are prepacked with high performance SepharoseTM based media with an affinity for human serum albumin (HSA) and IgG.

Legal Information

Sepharose is a registered trademark of Cytiva

SpinTrap is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva