Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose^® 6 Fast FLow with a chemically optimized linkage.Heparin is a mucopolysaccharide consisting of a repeating dimer of A-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-A-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.

Features and Benefits

20 mL column optimized for convenient scale-up purification . Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins. Simple operation with single pump configurations, as well as ÄKTA^® design systems. Excellent flow characteristics.

General description

HiPrep^™ Heparin FF 16/10 is prepacked with Heparin Sepharose^® 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Legal Information

HiPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol)