Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Sephacryl^® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.

Features and Benefits

Gel filtration columns for low to medium pressure AKTA systems. The HiPrep^™ Sephacryl^® series offer reproducible separations over a wide molecular weight range. Convenient, prepacked HiPrep^™ Sephacryl^® columns speed up protein purification. Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.

General description

HiPrep^™ 16/60 Sephacryl^® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.

Legal Information

HiPrep is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

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Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

gel filtration columns for low to medium pressure ÄKTA systems+AE14