Features and Benefits

Modified alkaline lysis procedure.

Anion exchange chromatography.

> 10 endotoxin units (EU) per µg of plasmid DNA.

Purification format is single-use, gravity flow illustra Fast-Flow plasmid 250 columns.

Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.

Provides plasmid DNA with excellent purity and low endotoxin levels.

High column capacity allows for a proportional yield for input sample volume of 25 to 50 ml (for high copy number plasmid) and up to 500 ml for low copy number plasmid.

> 70% (strain-dependent) of supercoiled pDNA.

General description

illustra plasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 ml for high copy number plasmid DNA and up to 500 ml for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA. The kit contains fast-flow plasmid 250 columns, resuspension, lysis, neutralization, wash, elution, and TE buffers, and a full protocol booklet with a detachable, quick reference protocol card.

Legal Information

illustra is a trademark of Cytiva