Analysis Note

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Application

illustra^™ GFX^™ 96 Purification Kit utilizes glass fiber matrix technology in a 96-well format for highly efficient purification of PCR products. Fragments from PCR are captured by the matrix in the presence of a chaotropic salt, and contaminants are conveniently removed by washing the matrix with a buffered ethanol solution.illustra^™ GFX^™ 96 PCR Purification Kit contains the following components in sufficient quantities: binding plates, wash plates, collection plates, color-coded bottles of capture buffer, wash buffer, and a choice of two elution buffers (Tris-HCl and sterile water), and an instruction booklet.

Features and Benefits

For the purification of up to 96 PCR products (0.1 to 10 kb) simultaneously in as little as 15 min. High yields of pure DNA recovered in a small volume of water or a Low ionic strength buffer. Typical recoveries are > 85% for PCR products 100 bp to 10 kb in length; salt removal typically ? 99%. Prepacked glass fiber matrix 96-well plates are validated for use with Macherey-Nagel NucleoVac 96 vacuum manifold. Avoids ethanol precipitations and hazardous organic extractions. Purified DNA is ready for use in most applications, including fluorescent sequencing, microarrays, labeling, hybridization, ligation, and transformation.

General description

Kit uses glass fiber matrix technology in a 96-well format for highly efficient purification of PCR products. DNA fragments from PCR are captured by the matrix in presence of a chaotropic salt and contaminants are removed by washing matrix.

Legal Information

GFX is a trademark of Cytiva

illustra is a trademark of Cytiva