Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose^® 6 Fast FLow (His MultiTrap^™ FF) or Ni Sepharose^® High Performance (His MultiTrap^™ HP). A protocol for His MultiTrap^™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.Loading unclarified samples directly to His MultiTrap^™ FF and His MultiTrap^™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap^™ FF and His MultiTrap^™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

Features and Benefits

Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins. Increased convenience and consistency with prepacked 96-well plates. No filtration needed - load unclarified sample directly, increase reproducibility, and save time. Use His MultiTrap^™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum. Easy scale-up to HisTrap^™ FF, HisPrep^™ FF 16/10 or HisTrap^™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

General description

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

Legal Information

HisPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

MultiTrap is a trademark of Cytiva

HisTrap is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)