G 418 disulfate salt, plant cell culture tested, BioReagent

Code: G1279-1G D2-231

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells tran...

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£200.00 1G

£240.00 inc. VAT

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OVERVIEW
SPECIFICATIONS
DOCUMENTS
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ATTRIBUTES

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 µg/mL or less while animal cells may require 300-500 µg/mL.

Caution

The product is stable for three years when stored at 2-8°C.

General description

Chemical structure: aminoglycoside

Packaging

250 mg in glass bottle

1, 5 g in glass bottle

Preparation Note

G418 powder is soluble in water at 100 mg/mL.

antibiotic activity spectrum	Gram-negative bacteria
application(s)	agriculture
color	white to off-white
form	powder
InChI key	UHEPSJJJMTWUCP-NKCAIAFTSA-N
InChI	1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;21-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
mode of action	protein synthesis \| interferes
potency	≥720 µg per mg (Dried basis)
product line	BioReagent
Quality Level	200
SMILES string	OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
solubility	100 mg/mL (H₂O clear)
storage temp.	2-8°C
technique(s)	cell culture \| plant: suitable