Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Flow cytometry/Cell sorting (1 paper)

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody is suitable for immunostaining of HEPC-CB2 and HEPC-CB.2 cell Lines (hematopoietic cells) and flow cytometry to evaluate the percentage of contaminating endothelia cells in characterization and decidualization of endometrial stromal cells.

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody has been used inDAB based-immunohistochemistrydirect immunofluorescent stainingfluorescence-activated cell sorting (FACS)flow cytometry

Biochem/physiol Actions

CD31 performs in homophilic and heterophilic cell-cell adhesion and cell signalling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix.

CD31 is also known as PECAM-1 (Platelet endothelial cell adhesion molecule-1) binds to glycosaminoglycans (GAG). Endothelial CD31 is phosphorylated on tyrosine and serine residues. CD31 is involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD31 is a single chain integral membrane glycoprotein member of the immunoglobulin superfamily of cell adhesion molecules with molecular mass of 130-140kDa. It has six Ig-like loops in the extracellular domain, a trans-membrane domain including a relatively long cytoplasmic domain. It is expressed abundantly on platelets, myeloid cells, B lymphocytes, certain T-lymphocyte cell subsets, bone marrow precursor cells, NK cells and localized to the intracellular junctions in monolayers of cultured endothelial cells.

Monoclonal Anti-CD31 (PECAM-1) is derived from the WM-59 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

human cell line RC-2A, originally derived from myeloid leukemia cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Specificity

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.5th Workshop: code No. P025

Target description

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.