Application

ExtrAvidin^® has been used to tetramerize aliquots to generate HLA-A2 tetramers.

Biochem/physiol Actions

ExtrAvidin^® is prepared from egg white avidin. It is a tetrameric protein containing four high affinity binding sites for biotin. It combines the high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. It binds biotin with the high affinity of egg white avidin, however, it does not exhibit the unwanted non-specific binding reported for egg white avidin at physiological pH, such as the staining of mast cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

A modified form of affinity purified egg white avidin.

Specificity

Binding Activity: at least 10 µg biotin per mg