Analysis Note

ControlRat E18 primary cortex cells

Application

Research CategoryNeuroscience

Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor^®555 Conjugate validated for use in ICC.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Immunogen

Epitope: C-terminus

Synthetic peptide corresponding to human Tubulin β-III.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tubulin β-III in rat E18 primary cortex cells.

Specificity

This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The unconjugated parent antibody (Cat. No. MAB1637) has an observed molecular weight at ~55 kDa