Features and Benefits
- Rapid - Optimized for fast reverse transcription real-time PCR
- Accurate quantification - For RNA from low copy targets
- Sensitive – unique buffer chemistry for highest specificity
- Flexible - Compatible with all fast cycling instruments
Instrument Compatibility ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, OneStep™ Plus, each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
The SensiFAST™ Probe Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit is designed for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe Hi-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR. The SensiFAST Probe Hi-ROX One-Step Kit consists of a 2x SensiFAST Probe Hi-ROX One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.
Volume BIO-77001: 100 x 20µl Reactions: 1 x 1ml BIO-77005: 500 x 20µl Reactions: 5 x 1ml
Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2 Plus separate vials of: Reverse Transcriptase, RNase inhibitor, DEPC-treated water
Kit Size: The kit size is based on a 20µl final reaction volume.