Analysis Note

ControlA549 cell lysate

Application

Anti-CYP24A1 Antibody is an antibody against CYP24A1 for use in 0.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Also known as Vitamin D(3) 24-hydroxylase, CYP24A1 is a member of the cytochrome P450 (CYP) family of enzymes. Most members of the CYP family can metabolize multiple substrates, and can catalyze multiple reactions. The CYP24A1 is a mitochondrial protein that starts the breakdown of 1,25-dihydrozyvitamin D3 by hydroxylation of the side chain to form calcitroic acid. This enzyme also has a role in calcium homeostasis and the vitamin D endocrine system.

Immunogen

KLH-conjugated linear peptide corresponding to human CYP24A1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in A549 cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected CYP24A1 in 10 µg of A549 cell lysate.

Specificity

Other Homologies: Bovine (92% sequence homology) and Rat (75% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed. Two isoforms are known to exist at 59 kDa and 51 kDa This antibody is expected to recognize both isoforms.