Analysis Note

ControlSH-SY5Y cell lysate

Application

Anti-C-X-C chemokine receptor type 5 (CXCR5) Antibody is an antibody against C-X-C chemokine receptor type 5 (CXCR5) for use in Western Blotting, IHC(P).

Immunohistochemistry Analysis: A 1:1,000 dilution from representative lot detected C-X-C chemokine receptor type 5 (CXCR5) in mouse frontal cortex neurons and mouse spleen lymphocytes.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

C-X-C chemokine receptor type 5 (CXCR5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15) is a G-protein coupled cytokine receptor that binds selectively to the CXCL13 (B lymphocyte chemoattractant) ligand, and mediates the development and function of the immune system. It is expressed in mature B cells, and facilitates the migration of B cells into follicles of the splenic white pulp, and enables deposition of antigen on follicular dendritic cells. It is also involved in the development of lymph nodes and Peyer's patches. Previous studies have reported that the expression of CXCR5 increases in rheumatoid arthritis.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human C-X-C chemokine receptor type 5 (CXCR5).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in SH-SY5Y cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected C-X-C chemokine receptor type 5 (CXCR5) in 10 µg of SH-SY5Y cell lysate.

Specificity

This antibody recognizes the cytoplasmic domain of C-X-C chemokine receptor type 5 (CXCR5).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~51 / 38 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing with molecular weights ~42 and ~37 kDa The calculated molecular weight of this protein is 42 kDa, but can be observed at ~51 kDa due to high glycosylation.