Analysis Note

ControlBovine calf lens, human kidney and calf kidney tissue lysate

Application

This Anti-alpha B-crystallin Antibody is validated for use in WB, IH(P) for the detection of alpha B-crystallin.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in rat lens fiber tissue. A 1:2,000 dilution from a representative lot detected Alpha-crystallin B chain in strong rat lens fiber tissue (data not provided). A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in strong muscle fiber tissue (data also not provided). It is recommended to use a 1:2,000 dilution only for strong rat lens fiber tissue.
Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of human kidney and calf kidney tissue lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus human alpha B-crystallin.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluted by Western Blot in bovine calf lens tissue lysate.

Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of bovine calf lens tissue lysate.

Specificity

This antibody recognizes the N-terminus of alpha B-crystallin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~20 kDa observed. Uncharacterized bands at ~37 and 43 kDa may be observed in some cell lyates.