Application

Research CategoryNeuroscience

Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, WB, IH.

Immunohistochemistry: 5-10µg/mL (see suggested protocol).

Immunoblotting: 5-10µg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000

ELISA: 5-10µg/mL

BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.



Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurochemistry & NeurotrophinsNeuroinflammation & Pain

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant Human Brain Derived Neurotrophic Factor (BDNF).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1534P

Physical form

Immunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 µL of sterile distilled water (1 mg/mL).

Format: Purified

Specificity

Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 1% cross-reactivity against NGF, NT3 or NT4.

Storage and Stability

Maintain lyophilized material at -20 to -70°C for up to one year after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Glycerol (ACS grade or better) can be added (1:1) for additional stability.