Application

Proteins from lysed Leishmania amazonensis cells were analyzed by western blot using HRP-conjugated goat anti-rabbit Fc specific IgG as the secondary antibody.

Anti-rabbit IgG (whole molecule), F(ab′)₂ fragment-peroxidase antibody can be used in immunoprecipitation and western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule), F(ab′)₂ fragment-peroxidase antibody can be used to detect immune-complexes. The product can also be used in direct ELISA. Goat anti- rabbit IgG (whole molecule), F(ab′)₂ fragment-peroxidase antibody reacts specifically with all rabbit immunoglobulins.

Immunogen

Purified rabbit IgG.

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).