DTSSP (33-DITHIOBIS(SULFOSUCCINIMIDYL

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Features and Benefits

Reactive groups: sulfo-NHS ester (both ends) Reactive towards: amino groups (primary amines) Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5 Spacer arm also easily cleaved with reducing SDS-PAGE sample loading Cleavable crosslinker allows separation of crosslinked products Water-soluble; compare with DSP Membrane-impermeable, allowing for cell surface labeling

General description

DTSSP is 3,3′-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.